Regional distribution from CO and you may GC situations
The extreme type in the proportion out of CO and you will GC situations observed along chromosomes using the negative matchmaking ranging from CO and GC costs therefore appear to be contradictory with the “relying model” while support a far more dynamic that associated with an adjustable DSB repair path otherwise DHJ solution around the genomes
It is also fascinating to note your seen activities regarding CO and GC distribution together chromosomes can tell us regarding the models recommended to describe chiasma disturbance. The fresh new “depending design” assumes you to double-string vacation trips occur individually, and that a fixed and you will system-particular count (m) regarding noncrossovers (GC incidents) are present anywhere between nearby crossovers , . A later extension of model integrated the possibility of a fraction off meiotic crossovers associated with the a moment path which is perhaps not at the mercy of disturbance .
At a 100-kb scale, we have shown that CO, and to a much lesser degree GC, are not randomly distributed across chromosomes. five hundred and GC500; see above). We found that the distribution of CO and GC events is not random in terms of intergenic/genic sequences, with a significant tendency to be located within genic sequences (P<0.00001, Figure 10A; see Materials and Methods for details). This excess is mostly due to GC500, with a highly significant preference for genic regions (P<0.00001) while CO500 show no preference or avoidance (P>0.40). The differential distribution of GC and CO when looking at genic and intergenic sequences is consistent with the heterozygosity-dependent GC?CO repair of DSB proposed above, given that intergenic sequences have higher levels of heterozygosity than genic sequences. Overall, our data suggest a higher probability of DSBs within annotated transcriptional units.
Analyses based on 1,909 and 3,701 CO and GC events delimited by 500 bp or less (CO500 and GC500). (A) Frequency of recombination events (CO or GC) within genic sequences. Probability [P (Freq. Observed
To analyze new shipment out-of CO and you may GC situations within a great so much more regional scale (the level of solitary genetics) i again focused on the five,610 CO and you will GC events delimited by the five hundred bp or shorter (CO
In yeast, some DSBs do not require transcriptional activity but depend on the binding of transcription factors, thus predicting an accumulation of recombination events near promoter regions. Alternatively, transcription may alter local chromatin structure, increasing the likelihood of DSB formation along the transcript unit ( and references therein). We therefore investigated the distribution of GC events along these sequences. We observe that the median position of GC500 is +910 from the transcription start site (TSS), close to the median midpoint of all D. melanogaster transcripts (+1,058). A split of transcripts into short (<2.5 kb) and long (>2.5 kb) shows the median GC500 position shifting significantly relative to the TSS (from +556 in short transcripts to +3588 in long transcripts; Mann-Whitney test U = 51,192, P<1?10 ?12 ). Moreover, the relative position of GC500 events along transcript sequences is uniform (Figure 10B), indicating that DSBs are not strongly associated with the binding of transcription factors. This latter result is also consistent with analyses of recombination at the rosy locus, where recombination is initiated throughout the gene . Altogether, our results favor a model where increased chromatin accessibility contributes to the definition of DSB sites in Drosophila, probably associated with transcriptional processes. Note that the preponderance of GC over CO events in many species, and the difference in their physical location across the genome, may limit analyses trying to assess the role of chromatin accessibility on DSB formation and their genomic distribution when using only data associated with COs.